Human intestinal organoid model

The human intestinal organs serve several functions including acting as a barrier, aiding in digestion and absorption, and facilitating movement. Moreover, they play a crucial role in regulating the physiological state of the entire body by metabolizing drugs and communicating with intestinal microorganisms as well as other organs.

In comparison to the conventional Caco-2 Transwell model, Daxiang's intestinal model utilizes human intestinal organoids and incorporates the necessary fluid shear force found in the tissue microenvironment, resulting in a model that more closely mimics the normal physiological state of humans at the cellular level. This makes it an excellent tool for pharmaceutical ADME studies.

Applications
  • Drug absorption/metabolism
    /transport evaluation
  • Intestinal inflammation model construction
  • Gut flora research
Sample data
Recapitulation of physiological state of the human body
On the traditional Transwell, the cells only maintain a dense cobblestone structure. However, with the support of dynamic fluid, intestinal epithelial cells can now form a more complex 3D structure that closely resembles the human body. Additionally, the intestinal epithelial monolayer TEER gradually increases over time and remains stable after 10 days of operation.
Characterization of Colon Organoid Differentiation Markers
Intestinal differentiation marker proteins Villin, Mucin 2 and glycocalyx were expressed and distributed on the apical side of the colon.
Stronger intestinal absorption
Under fluid stimulation, the P-gp transporter has a stronger function. After culturing for 24 days, fluorescence imaging was performed with the presence of a P-gp inhibitor. A strong fluorescence accumulation of Rhodamine was observed in the cells.
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