Assessment of the ability to penetrate the blood-brain barrier

Metabolic stability (microsomes/S9/hepatocytes/plasma))

P450 enzyme inhibition (including TDI)

P450 enzyme inductio

P450 Enzyme Phenotyping (Recombinase/Chemical Inhibitors)

Protein binding (plasma/tissue/microsome)

Red blood cell/plasma partition ratio

Caco-2 permeability

 (P-gp/BCRP/OATs/OCTs/OATPs) / • Efflux and uptake transporters (P-gp/BCRP/OATs/OCTs/OATPs) 

In vitro metabolite identification

Capture of GSH reactive metabolites